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Theory And Mechanisms Of Staining

 Theory and Mechanisms of Staining (9815-08) Review the theory and mechanisms of staining, dye classification and tissue demonstration methods and improve your skills and the quality of prepared material.

Code 9815-08
Level Advanced

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Additional Details

PEP hours: 12
CPS credits: 0.5
Level: Advanced
Course Type: Express

Start Date: Upon registration
Completion: Up to 52 weeks
Delivery: PDF via email

Prerequisites: None
Textbook: N/A
Equipment: Computer with Internet is required

Learning Outcomes:

  • Define the term resonance system in relation to dye chemistry
  • Define the following terms in relation to dye structure and classification: chromophore (quinoid ring, azo coupling, nitro grouping), chromogen, auxochrome (hydroxyl, amine, carboxyl, sulphonic)
  • Relate acid, basic and neutral dyes to their staining properties
  • Discuss physical theories of staining: selective solubility, adsorption, porosity
  • Describe chemical theories of staining: salt linkage, hydrogen bonding, metachromasia, leukostaining (leucostaining), metallic impregnation, histochemical reactions
  • Describe proper storage of dyes and indicate criteria
  • Define colour index number
  • Differentiate between progressive and regressive staining and state advantages and disadvantages of each
  • Classify dyes according to origin and examples of each
  • Explain the principle and use of direct and indirect staining
  • Describe use of accelerators and accentuators. Define the terms.
  • State principle and application of argyrophil and argentaffin reactions
  • Name three (3) chromophoric groups found in many commonly used stains, show the basic chemical structure and name at least one dye that contains the group
  • Define: Basophilic tissue, Acidophilic tissue
  • Define: Mordant, Lake, Accentuator, Accelerator, Metachromasia, Leuco compound, Chromotrope, Orthochromasia

Author/Instructor: Masaye Tanaka, MLT, ART, BSc, MEd
Version Date: September 2008