Routine And "Not-So-Routine" Methods In Transfusion Science
Routine and "Not-So-Routine" Methods in Transfusion Science (9834-10)
Review basic principles in compatibility testing and explore the advantages as well as the pitfalls of both routine and special methods. Study all the crucial steps in pretransfusion compatibility testing from identification of patient and sample to the actual crossmatch and preparation of blood units to leave the laboratory. Identify unexpected red cell alloantibodies by recognizing reaction patterns, excluding antibodies with panel cells and phenotyping red cells. Relate theory, clinical observations, and practical approaches to the investigation of a positive direct antiglobulin test.
PEP hours: 11
CPS credits: 0
Course Type: Express
Start Date: Upon registration
Completion: Up to 52 weeks
Delivery: PDF via email
Equipment: Computer with Internet is required
- State essential steps in pretransfusion compatibility testing.
- Discuss functions and limitations of pretransfusion compatibility testing.
- Recognize the importance of proper patient and sample identification.
- Discuss factors affecting ABO/Rh test results.
- List minimum requirements of pretransfusion antibody detection.
- Select appropriate ABO compatible units when recipient’s ABO identical donors are in short supply.
- Discuss advantages and disadvantages of including the indirect antiglobulin test in the major crossmatch.
- Describe the minimum requirements of electronic (computer-assisted) crossmatch.
- Use same reaction conditions to identify antibody as detected in antibody screen.
- State the three essential steps and their limitations in antibody identification.
- Understand the effects of proteolytic enzymes on blood group antigens.
- Be aware of the effects of reducing agents such as ZZAP and AET on Kell and Lutheran blood group system antigens.
- Provide a detailed list of blood group antigens that are weakly expressed on cord red cells.
- Use appropriate blood group substances in neutralization assays for antibody investigation.
- Be aware of application and interpretations of serological techniques such as titration, serum acidification, adsorption and elution in antibody identification.
- Interpret and discuss the clinical significance of a positive direct antiglobulin test.
- Classify immune hemolytic anemia.
- Be aware of cold agglutinin specificities other than Ii.
Author/Instructor: Eric Ching, ART, MT(ASCP)SBB
Version Date: January 2010