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Neisseria And Moraxella

 Neisseria and Moraxella (9821-12) Learn the morphology, growth requirements, identification tests and susceptibility patterns, and pathogenicity in order to better differentiate Neisseria species including N. gonorrhoeae, N. meningitides and Moraxella catarrhalis.

Code 9821-12
Level Basic

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Additional Details

PEP hours: 11
CPS credits: 0
Level: Basic
Course Type: Express

Start Date: Upon registration
Completion: Up to 52 weeks
Delivery: PDF via email

Prerequisites: None
Textbook: N/A
Equipment: Computer with Internet is required

Learning Outcomes:
Neisseria gonorrhoeae

  • Describe typical cellular morphology in direct smears and cultures.
  • State the significance of finding intracellular bacteria in urethral exudate.
  • State the growth requirements
  • Explain how auxotyping is performed and why this may be done.
  • Name suitable media for isolation. Explain the usual reason why a strain of N. gonorrhoeae may fail to grow on selective media.
  • Explain the difference between a holding and growing transport medium.
  • Describe culture techniques used to enhance growth of N. gonorrhoeae in culture media.
  • State the criteria used to establish the genus Neisseria.
  • List the five types of tests used for identification and know the reactions for N. gonorrhoeae
  • Name the two types of serological tests available for identification of N. gonorrhoeae.
  • Describe the advantage of a gene probe technique in diagnosis of gonorrhea.
  • Describe the changing susceptibility pattern for N. gonorrhoeae. Describe the two resistance mechanisms to penicillin and state how each is detected.
  • Describe clinical infections caused by N. gonorrhoeae.

Neisseria meningitidis

  • Describe typical cellular morphology.
  • Describe growth requirements and typical colonies on blood agar.
  • State reactions in carbohydrate utilization tests. Name two other test systems that may be used for identification.
  • State the usual susceptibility to penicillin.
  • State where N. meningitidis is found in carriers.
  • Name the two infections commonly caused by N. meningitidis.
  • Describe typical findings in spinal fluid from cases of meningococcal meningitidis and state how antigen can be detected directly from spinal fluid.

Neisseria species

  • Describe typical cellular morphology.
  • State the growth requirements
  • Describe colonial morphology typical of Neisseria species.
  • State when definitive identification is required and how this is accomplished (not specific reactions).
  • For N. lactamica, know what type of media it grows on and the reactions in the carbohydrate utilization tests.
  • Explain how N. polysaccharea and N. subflava could be confused with N. meningitidis and how they may be differentiated using biochemical tests.
  • Explain why N. cinerea may be wrongly identified as N. gonorrhoeae and how to avoid this problem.
  • State where Neisseria species are found as normal flora and types of infections reported.

Moraxella catarrhalis

  • State the three names previously used for this organism.
  • Describe typical cellular morphology.
  • Describe growth requirements. Describe typical colonies on blood agar.
  • State reactions in carbohydrate utilization tests and state how differentiation from Neisseria species is accomplished.
  • State how and why beta-lactamase testing should be done.
  • List common infections caused by M. catarrhalis.

Oxidase Test

  • Outline the principle of the test.
  • Name the active ingredient in oxidase reagent.
  • Explain how the reagent is stabilized and how this affects stability and sensitivity.
  • Describe the filter paper method
  • State when quality control organisms should be tested and name suitable control organisms.

Author/Instructor: Helen Smith, MLT
Version Date: January 2012