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Miscellaneous Gram Positive Rods

 Miscellaneous Gram Positive Rods (9828-12) Improve your ability to isolate, identify, differentiate and be better prepared to deal with Bacillus anthracis, Corynebacterium diphtheria and Listeria.

Code 9828-12
Level Basic

Member Price

$0

Non-Member Price

$135

 

Additional Details

 
PEP hours: 10
CPS/ART credits: 0
 
Learning Outcomes:
Facultative and Aerobic Spore Forming Rods
·       State the criteria that place an organism in the genus Bacillus.
·       Name the major pathogen of the genus.
·       Explain why B. anthracis is dangerous to work with in the laboratory and state where cultures should be handled.
·       Describe the cellular morphology and growth requirements of B. anthracis.
·       Describe typical colonies of B. anthracis on blood agar.
·       Describe three criteria useful for preliminary differentiation of B. anthracis from other Bacillus species.
·       Describe the pathogenicity of B. anthracis in animals and explain how humans may be infected.
·       State the usual significance of Bacillus species in cultures.
·       List infections that may be caused by Bacillus species.
·       Describe cellular morphology of Bacillus species.
·       Explain how the KOH test and vancomycin susceptibility may be used to help interpret the Gram reaction.
·       Describe food poisonings caused by Bacillus cereus.
·       Name the organism used as a biological control for the autoclave and state the temperature required for growth of this organism.
 
Corynebacterium and Similar Gram Positive Bacilli
  • List the common characteristics of the genus Corynebacterium.
  • Explain the general meaning of the term "diphtheroid".
  • Describe the frequency of isolation of C. diphtheriae in Canada.
  • Describe the cellular morphology of C. diphtheriae.
·         Describe volutin granules.
·         Describe the selective media used to isolate C. diphtheriae.
  • Describe typical colonies of C. diphtheriae on blood agar.
  • State how genus identification for Corynebacterium is established, how the species diphtheriae is identified and what additional testing is required for C. diphtheriae.
  • Name the type of test used for the in vitro identification of toxin.
  • Describe the clinical symptoms of diphtheria and the role of toxin.
  • Name the usual site of infection as well as other possible sites.
  • State how immunization is accomplished.
  • Describe the need for screening for C. diphtheriae in Canada.
·       Describe the clinical significance of Corynebacterium ulcerans and how to differentiate it from C. diphtheria.Describe the type and incidence of Corynebacterium pseudotuberculosis infections and how to differentiate from C. diphtheriae:
·       State where diphtheroids are found as normal flora and the significance in blood cultures.
·       Describe typical colonies on blood agar and typical cellular morphology.
·       Explain when and how definitive identification is made.
·       Describe the clinical significance, cellular morphology and susceptibility pattern of Corynebacterium jeikeium
·       Describe the clinical significance of Arcanobacterium haemolyticum and how to differentiate it from streptococcus species.
·       State the clinical significance of Actinomyces pyogenes.
·       Describe the cellular and colonial morphology of Rhodococcus equi and its pathogenicity.
 
Listeria monocytogenes
·       Name the two species of Listeria isolated from man and state which one is a known human pathogen.
·       Describe the cellular morphology of L. monocytogenes.
·       State the optimum atmosphere and temperature for growth.
·       State when selective media would be required and explain the principle of cold enrichment.
·       Describe typical colonies on blood agar.
·       State the result for L. monocytogenes in the following tests:
·         Catalase
·         Slide motility
·         Media motility
·         Esculin hydrolysis
·         CAMP reaction with S. aureus and R. equi
·       Describe the epidemiology of infections in man.
·       Describe infections in the neonate.
·       List infections caused in adults and predisposing factors.
 
Erysipelothrix
·       State the reservoir of Erysipelothrix rhusiopathiae, how man is infected and the type of infection seen in man.
·       Describe specimens suitable for culture and appearance of E. rhusiopathiae in each.
·       Name a suitable medium for isolation and state optimum environment for growth.
·       Describe colonies on blood agar and typical findings in Gram stains.
·       State reaction for catalase and motility and explain the importance of H2S production in identification.
 
Gardnerella
·       Name the two genera previously used for Gardnerella.
·       Describe the cellular morphology in cultures and direct smears.
·       State the optimum atmosphere and temperature for isolation.
·       State the effect of sodium polyanethol sulfonate on growth.
·       Name two nonselective and one selective medium for isolation.
·       Describe typical colonies on medium containing human blood.
·       State the criteria for presumptive identification.
·       List the three biochemical tests commonly used to confirm identification.
·       Name the antimicrobial commonly used for treatment of vaginosis and state if susceptibility testing is required.
·    Describe bacterial vaginosis.
·    Describe other infections that may be caused by G. vaginalis.
 
Instructor: Helen Smith, MLT
Prerequisites: None
Textbook: N/A
Equipment: Computer with Internet is required
Start Date: Upon registration
Completion: Up to 52 weeks
Version Date: January 2012