Leukocytes - Measurement Of Neutrophil Function
Leukocytes - Measurement Of Neutrophil Function (9829-09)
Review laboratory procedures within a practical context and improve your ability to make decisions regarding identifying neutrophilic functional defects.
PEP hours: 11
CPS credits: 0.7
Course Type: Express
Start Date: Upon registration
Completion: Up to 52 weeks
Delivery: PDF via email
Equipment: Computer with Internet is required
- Summarize granulopoiesis and neutrophil development.
- Explain the significance of neutropenia with regard to neutrophil function
- Describe the correct way to collect blood and prepare a neutrophil suspension of viable cells.
- Explain the principles of separating the individual cellular elements of blood.
- Identify viable from non-viable separated neutrophils.
- Explain how in vitro testing can determine if neutrophils have the normal capacity for phagocytosis.
- Present a method to demonstrate the ability of neutrophils to kill engulfed bacteria.
- Tabulate the disorders encountered with defective microbial killing ability.
- Describe the important features of Chronic Granulomatous Disease
- Describe the nitroblue tetrazolium test ( NBT ) for the diagnosis of chronic granulomatous disease
- List all of the situations where the NBT test may be helpful in various clinical situations.
- Briefly explain the concept of flow cytometry as related to neutrophil function testing
- Explain the principle behind the Rebuck skin window test performed in vivo compared to the Boyden chamber technique which is an in vitro test.
- Describe the tests that are able to measure neutrophil chemotaxis.
- Describe which morphological appearances might be helpful with regard to neutrophil function.
- Outline how all of the neutrophil function test may be applied diagnostically.
- Present the limitations of neutrophil function tests.
Author/Instructor: John Chapman, FCSMLS, FIMLS, CLSp(H)
Version Date: November 2009