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Hemolytic Disease Of The Fetus And Newborn

 Hemolytic Disease of the Fetus and Newborn (9860-10) Learn how prenatal testing can prevent or detect risk to this disease and how postnatal investigations are used to detect the presence and severity of HDFN. You will learn more about the mechanisms of HDFN and the laboratory tests such as hemoglobulin, bilirubin, ABO grouping, D typing, antibody screens, DAT, IAT, Kleihauer-Betke and rosetting tests, that support the clinician's efforts to deal with this disease. Version Date: August 2010

Code 9860-10
Level Basic

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Additional Details

PEP hours: 15
CPS credits: 0
Level: Basic
Course Type: Express
Start Date: Upon registration
Completion: Up to 52 weeks
Delivery: PDF via email
Prerequisites: None
Textbook: N/A
Equipment: Computer with Internet is required
Learning Outcomes:
  • Define the term hemolytic disease of the fetus and newborn (HDFN).
  • Describe three possible causes of maternal immunization in HDFN.
  • Briefly describe the pathophysiology of HDFN.
  • Explain why ABO HDFN is usually milder than Rh HDFN.
  • State five factors affecting the severity of HDFN.
  • List five considerations that should be given when a prenatal sample tests positive for an unexpected antibody.
  • Understand basic principles of clinical and laboratory methods used to monitor patients at risk for HDFN.
  • Explain why titration of alloantibody is only semiquantitative at best.
  • Describe specific donor requirements for intrauterine and exchange transfusion and explain why each requirement is necessary.
  • Describe compatibility testing for an exchange transfusion, including specimen requirement and testing done on donor and recipient
  • Describe Rh immune globulin
  • Describe the principle and the appropriate use of the indicator cell rosette test and the Kleihauer-Betke test for estimation of volume of fetomaternal bleed.
  • State the indications for cord blood testing, explaining the possible causes of false positive or negative reactions in ABO/Rh testing.
  • Explain how and why the indirect antiglobulin test is used on infant's serum or plasma for the diagnosis of ABO HDFN.
  • Describe the follow-up investigation of a positive DAT on cord red cells.
Author/Instructor: Eric Ching, ART, MT(ASCP)SBB
Version Date: August 2010