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Hematoxylin And Eosin (H&E) Stain

 Hematoxylin And Eosin (H&E) Stain (9816-08) Reinforce your understanding of the H&E stain by examining this stain in detail in terms of theory, practice, category differentiation and trouble-shooting "when things go wrong".

Code 9816-08
Level Advanced

Member Price

$65

Non-Member Price

$110

 

Additional Details

PEP hours: 9
CPS credits: 0.4
Level: Advanced
Course Type: Express

Start Date: Upon registration
Completion: Up to 52 weeks
Delivery: PDF via email

Prerequisites: None
Textbook: N/A
Equipment: Computer with Internet is required

Learning Outcomes:

  • Describe the stain theory principles involved in the H&E stain.
  • Name the source of the natural stain "hematoxylin".
  • Differentiate between hematoxylin and hematein.
  • Name 4 chemicals used as mordants.
  • Define "alum".
  • Describe the colour produced by the different mordants with hematoxylin.
  • Name four (4) chemicals used as oxidants.
  • Define oxidation ("ripening") of hematoxylin.
  • Define/discuss and distinguish between regressive and progressive hematoxylin staining
  • Discuss blueing of hematoxylin stained sections under the following headings:
  1. Principle
  2. Mechanism
  3. Result
  4. Reagents used.
  5. pH

 

  • Classify the dye group to which eosin belongs
  • Differentiate the dye as anionic or cationic
  • Name three (3) other commonly used dyes that belong to the same dye category as eosin.
  • Differentiate between eosin Y and eosin B.
  • Discuss properties, advantages, disadvantages, use and chemical composition of Harris, Mayer, Weigert, Verhoeff and Gill hematoxylin.
  • State a cause and suggest corrective technique for the following problems that may occur with hematoxylin staining:
  1. Uniformly weak staining of nuclei
  2. Nuclei dark blue with poor detail and cytoplasm is grey-blue.
  3. Dark granules (purple) have precipitated on the sections
  4. Nuclei are stained dull red, or brown
  • State a cause and suggest corrective technique for the following problems that may occur during eosin staining:
  1. Uniformly weak staining of cytoplasm
  2. Nuclei dark blue with poor detail and cytoplasm is grey-blue
  3. Dark staining with eosin that obscures the nuclear stain
  4. Nuclei are stained dull red, or brown
  • Define colour index number and state the purpose for its use.

Author/Instructor: Masaye Tanaka, MLT, ART, BSc, MEd
Version Date: September 2008