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Fixation Practices

 Fixation Practices (9893-09) Learn how to categorize different types of fixing agents; to elaborate on concepts in fixation and preservation of cellular elements for different types of analysis; and to utilize principles of tissue/cell preservation and fixation in order to effectively demonstrate specific components of cells and tissues.

Code 9893-09
Level Basic

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Additional Details

PEP hours: 11
CPS credits: 0
Level: Basic
Course Type: Express
Start Date: Upon registration
Completion: Up to 52 weeks
Delivery: PDF via email
Prerequisites: None
Textbook: N/A
Equipment: Computer with Internet is required
Learning Outcomes:
  • Describe basic structure and reactions of protein in relation to fixation
  • Define purpose/function of fixation
  • Identify chemical structure of acetone, formaldehyde, ethanol, formic acid, picric acid, methanol, acetic acid, mercuric chloride, glutaraldehyde, osmium tetroxide, potassium dichromate, glyoxal
  • Describe features of fixing agents
  • Define preservation and differentiate from fixation.
  • Identify fixation/fixative artifacts
  • Describe the effects and optimization of temperature, volume of fixative, osmolality, concentration, time of exposure and [H+] concentration on fixation of tissue
  • Describe how the size of tissue affects fixation
  • State the optimal thickness of tissue for paraffin processing for light microscopy
  • State the optimal thickness of tissue for electron microscopy
  • Differentiate between non-coagulant and coagulant fixative
  • Describe the mode of action and general classification, advantages, disadvantages and safety precautions associated with: formaldehyde, ethanol and alcohol solutions, picric acid, osmium tetroxide, mercuric chloride
  • Describe conditions/analyses when/where fresh, unfixed tissue is required
  • Define/Describe the use of post-fixation
  • Discuss the theories regarding mechanisms of various types of fixatives
Author/Instructor: Masaye Tanaka, MLT, ART, BSc, MEd
Version Date: December 2009