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Fastidious Gram Negative Bacilli

 Fastidious Gram Negative Bacilli (9820-11) Learn about the classification and nomenclature, morphology and growth requirements, identification and susceptibility, pathogenicity and relationship to differentiation strategies for Haemophilus, Bordetella, Pasteurella, Gardnerella and Capnocytophaga.

Code 9820-11
Level Basic

Member Price

$65

Non-Member Price

$110

 

Additional Details

PEP hours: 11
CPS credits: 0
Level: Basic
Course Type: Express

Start Date: Upon registration
Completion: Up to 52 weeks
Delivery: PDF via email

Prerequisites: None
Textbook: N/A
Equipment: Computer with Internet is required

Learning Outcomes:

  • State the criteria originally used to place an organism in the genus Haemophilus.

Haemophilus influenzae

  • Describe the cellular morphology.
  • Describe the growth requirements:
  • Describe colonial morphology on blood agar with a staph streak and on chocolate agar.
  • State the criteria necessary to establish the genus Haemophilus.
  • State how to differentiate the different species of Haemophilus.using hemolysis, growth factor requirements and porphyrin test.
  • Explain why biotyping and serotyping would be carried out and state which serotype is the most virulent.
  • State the approximate rate of resistance to ampicillin, describe the two mechanisms responsible for the resistance and how each is detected.
  • Name a rapid test for chloramphenicol resistance.
  • Explain why childhood infections due to this organism are decreasing in number.
  • Name the two invasive infections found in children and describe typical findings in spinal fluid from a case of meningitis.

Haemophilus aegyptius

  • Explain the taxonomic relationship with H. influenzae.
  • Describe two acute infections caused by H. aegyptius.

Other Haemophilus species

  • Describe the clinical significance of H. parainfluenzae, H. haemolyticus and H. parahaemolyticus.
  • State the atmosphere required for isolation of H. aphrophilus and the type of specimen where it may be found as a pathogen.
  • Describe the cellular morphology of H. ducreyi in direct smears and in cultures.
  • State the pathogenicity of H. ducreyi.

X and V Growth Factor Requirements

  • State the criteria the test medium must meet and name the recommended medium.
  • State how the test medium should be inoculated, how carryover of growth factors in the inoculum would affect results and how to avoid this.
  • Describe how to interpret results.

Porphyrin Test

  • State the principle of the test including the substrate used.
  • State how the test is inoculated and describe two methods of detecting porphyrins.
  • State how to interpret test results.
  • Explain how the test interpretation is affected by:
  • Testing bacteria belonging to another genus
  • Using non-viable bacteria
  • Using too light an inoculum

Bordetella

  • Name the species most commonly infecting man.

Bordetella pertussis

  • Describe typical cellular morphology.
  • Describe how diagnosis may be made using direct smears.
  • Name the site that should be sampled for culture and explain how the specimen is obtained.
  • Name and describe a suitable transport medium.
  • Describe the growth requirements. Name two media used for isolation of B. pertussis, compare the shelf life and name the antibiotic recommended for these media.
  • State the usual time required for visible colonies to form.
  • State the criteria indicating the genus Bordetella.
  • State how to differentiate B. pertussis from B. parapertussis and B. bronchiseptica.
  • Name the disease caused by B. pertussis and describe the symptoms.
  • Describe the clinical significance of B. parapertussis and B. bronchiseptica.

Capnocytophaga

  • Describe typical cellular morphology.
  • State suitable media and atmosphere for growth.
  • Describe typical colonies.
  • Describe laboratory findings that indicate Capnocytophaga.
  • State how complete species identification is carried out.
  • Describe the pathogenicity of C. canimorsus and state the usual source of the bacteria.

Actinobacillus

  • Describe typical cellular morphology.
  • State suitable media and atmosphere for growth.
  • Describe typical colonies.
  • Describe laboratory findings that indicate Actinobacillus
  • Describe the pathogenicity of Actinobacillus and state the usual source of the bacteria.

Eikenella

  • Describe typical cellular morphology.
  • State suitable media and atmosphere for growth.
  • Describe typical colonies.
  • Describe laboratory findings that indicate Eikenella
  • Describe the pathogenicity of Eikenella and state the usual source of the bacteria.

Kingella

  • Describe typical cellular morphology.
  • State suitable media and atmosphere for growth.
  • Describe typical colonies.
  • Describe laboratory findings that indicate Kingella.
  • Describe the pathogenicity of Kingella and state the usual source of the bacteria.

Cardibacterium

  • Describe typical cellular and colonial morphology.
  • Describe pathogenicity

Pasteurella

  • Name the species most commonly isolated from man.
  • Name the genus to which some Pasteurella species are being moved.

Pasteurella multocida

  • Describe typical cellular morphology.
  • Describe growth requirements:
  • Describe typical colonies on blood agar.
  • State criteria that indicate a possible P. multocida.State the usual source of the bacteria infecting man and describe clinical infection.

Author/Instructor: Helen Smith, MLT
Version Date: December 2011