Enterobacteriaceae
Enterobacteriaceae (9830-11)
Reinforce your knowledge and skills as you use nomenclature, cultural characteristics, identification techniques and pathogenicity to differentiate Escherichia, Shigella, Salmonella, Enterobacter and other clinically significant isolates.
Member Price
$0
Non-Member Price
$135
Additional Details
PEP hours: 8
CPS/ART credits: 0
Learning Outcomes:
Enterobacteriaceae
· List the common characteristics of the family Enterobacteriaceae.
· List the three Enterobacteriaceae most commonly isolated from clinical specimens.
Escherichia
· Describe typical colonies on blood and MacConkey agar.
· State typical reactions for:
▪ Glucose
▪ Lactose
▪ TSI
▪ ONPG
▪ Indole
▪ Voges-Proskauer
▪ Citrate
▪ Motility
▪ Lysine
· State how rapid identification of E. coli may be carried out.
· List infections commonly caused by E. coli.
· List the four types that cause enteric disease
· Know the significance of an ESBL producer.
Shigella
· Name the genus most closely related to Shigella.
· Describe colonies on MacConkey agar.
· State the suitability of SS, XLD, HE, tetrathionate, selenite and GN broth for isolation of Shigella.
· State typical reactions for:
▪ Motility
▪ Lactose
▪ Phenylalanine deaminase
▪ Glucose
▪ Sucrose
▪ Urease
· State how species identification is established.
· Describe the procedure for serogrouping.
· Explain why serotyping would be done.
· Describe the pathogenicity.
Edwardsiella
· State reactions that would distinguish Edwardsiella from E. coli and from Salmonella species.
· State the clinical significance.
Salmonella
· Describe typical colonies on Mac, SS, XLD, SS, HE and bismuth sulfide media.
· State typical reactions for:
▪ Glucose
▪ TSI
▪ Motility
▪ Indole
▪ Citrate
▪ Phenylalanine deaminase
▪ Urease
▪ ONPG
▪ Lysine decarboxylase
· State reactions that may differentiate S. typhi from other Salmonella species.
· Outline the steps in serological identification.
· Describe the infection caused by S. typhi and state what specimens are best for diagnosis at various stages of disease.
· Describe the pathogenicity of the other Salmonella species and state the specimen required for diagnosis.
· State the usual bacterial reservoir of Salmonella species.
Citrobacter
· Compare reactions of Citrobacter freundii with those of Salmonella species in:
▪ TSI
▪ ONPG
▪ Lysine decarboxylase
· Describe the pathogenicity of Citrobacter
Klebsiella
· Name the two most commonly isolated species.
· Describe typical colonies on MacConkey agar and state the incidence of capsules.
· State typical reactions for K. pneumoniae and K. oxytoca for:
▪ Glucose
▪ Lactose
▪ TSI
▪ Motility
▪ Citrate
▪ Indole
▪ Ornithine decarboxylase
· List common infections and describe the role of Klebsiella in nosocomial infections.
Enterobacter
· Name the two species most commonly isolated from clinical material.
· Describe typical colonies of Enterobacter aerogenes and E. cloacae on MacConkey agar.
· State the colour of pigment produced by E. sakazakii and Pantoea (Enterobacter) agglomerans.
· State typical reactions of Enterobacter aerogenes and E. cloacae for:
▪ Glucose
▪ Lactose
▪ TSI
▪ Motility
▪ Citrate
▪ Indole
▪ Ornithine decarboxylase
· Describe the clinical significance of Enterobacter aerogenes, E. cloacae and E. sakazakii.
Hafnia
· State the genus most similar to Hafnia in biochemical reactions.
· Describe typical colonies on MacConkey agar. Compare Hafnia and Shigella in the following tests:
▪ TSI
▪ Motility
▪ Shigella Antisera
· State the clinical significance
Serratia
· Name the species most commonly isolated from clinical material.
· Describe the pigment occasionally seen.
· Describe colonies on MacConkey agar.
· State reactions of Serratia marcescens for:
▪ DNase
▪ Glucose
▪ Lactose
▪ Sucrose
▪ TSI
▪ Phenylalanine deaminase
▪ Arabinose
· Describe the pathogenicity of S.marcescens and its role in nosocomial infections
Proteus
· Name the three species recognized as human pathogens
· Describe typical colonies on blood, MacConkey and stool media
· State typical reactions for:
▪ Glucose
▪ Lactose
▪ Sucrose
▪ H2S
▪ TSI
▪ Phenylalanine deaminase
▪ Urease
▪ Indole
· State the significance in stool cultures
· Describe pathogenicity
Morganella
· Name the one species
· Describe colonies on blood and MacConkey agar
· State typical reactions for:
▪ Glucose
▪ Lactose
▪ Sucrose
▪ H2S
▪ TSI
▪ Phenylalanine deaminase
▪ Urease
· Describe the clinical significance
Providencia
· Name the two species most often isolated from clinical specimens
· Describe colonies on blood and MacConkey agar
· State typical reactions for:
▪ Glucose
▪ Lactose
▪ Sucrose
▪ H2S
▪ TSI
▪ Phenylalanine deaminase
▪ Urease
· Describe the clinical significance
Yersinia
· Name the three species that are well recognized as human pathogens.
· State the optimum temperature range for growth.
· Describe the suitability of the following media for isolation of Y. enterocolitica:
▪ Blood agar
▪ MacConkey agar
▪ SS agar
▪ Cold enrichment
▪ CIN agar
· Describe typical colonies of Y. enterocolitica on blood and CIN agar.
· List six characteristics that may indicate Y. enterocolitica.
· Describe the pathogenicity of Y. pestis, Y. enterocolitica and Y. pseudotuberculosis.
Instructor: Helen Smith, MLT
Prerequisites: None
Textbook: N/A
Equipment: Computer with Internet is required
Start Date: Upon registration
Completion: Up to 52 weeks
Version Date: December 2011