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Connective Tissue

 Connective Tissue (9894-09) Review connective tissue and the various methods for demonstration. Strategies that will maintain reproducibility and consistency in staining results will also be discussed.

Code 9894-09
Level Basic

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Additional Details

PEP hours: 11
CPS credits: 0
Level: Basic
Course Type: Express
Start Date: Upon registration
Completion: Up to 52 weeks
Delivery: PDF via email
Prerequisites: Theory and Mechanisms of Staining (9815-08), Hematoxylin and Eosin (H&E) Stain (9816-08), Paraffin Tissue Processing (9822-08), Fixation Practices (9893-09)as well as recognize light microscope characteristics of normal tissue in H&E stained preparations, identify dyes using color index (CI) number and understand physicochemical stain theories
Textbook: N/A
Equipment: Computer with Internet is required
Learning Outcomes:
  • Describe connective tissue
  • Identify connective tissue microscopically
  • Identify and describe connective tissue fibers
  • Identify and describe connective tissue cells
  • Identify methods that demonstrate connective tissue
  • Name tissue controls that can be used to demonstrate connective tissue
  • Describe the fixative/fixation method that best preserves connective tissue components
  • Identify processing and tissue preparation to best preserve lipid.
  • Identify function(s) of the main reagents used in connective tissue demonstration methods
  • State the theory/principle of: elastic tissue demonstration methods, lipid demonstration, metachromasia, Romanowsky stains for hematopoetic cells, trichrome methods
Author/Instructor: Masaye Tanaka, MLT, ART, BSc, MEd
Version Date: December 2009