Body Fluids: Laboratory Methods
Body Fluids: Laboratory Methods (9825-12)
Learn tips for differentiating malignant cells from normal cells using techniques such as cell counting, film preparation, staining, evaluation and interpretation. Specimen collection, Cytospin preparation and confusing artefacts are also discussed.
PEP hours: 7
CPS credits: 0.28
Course Type: Express
Start Date: Upon registration
Completion: Up to 52 weeks
Delivery: PDF via email
Equipment: Computer with Internet is required
- Describe the technical aspects of specimen collections in order to have the ideal specimen for evaluation.
- Determine the appropriate method for cell counting
- Detail the methods for cell counting using both manual and automated cell counting methods.
- Describe the technique for preparing cytospin films using the cytocentrifuge.
- Outline the special tips and techniques available to enhance the quality of cytospin films.
- Describe the staining characteristics of cells commonly found in body fluids.
- Tabulate the method for staining films using the Papanicolaou stain.
- Outline the technique for staining films using a Wright-Giemsa stain.
- Identify the normal cells typically found in body fluids.
- Describe the appearance and normal occurrence of lymphoid, myeloid, mononuclear and lining cells.
- Describe the features of normal cells and compare them to typical features of malignant cells.
- Tabulate a scheme for differentiating normal from malignant cells.
- Describe in detail the common artefacts seen in cellular detail found in cytospin preparations.
Author/Instructor: John Chapman, FCSMLS, FIMLS, CLSp(H)
Version Date: November 2012