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Body Fluids: Cerebrospinal Fluid

 Body Fluids: Cerebrospinal Fluid (9804-11) Review the many laboratory techniques for evaluating CSF including routine procedures and new technologies. This module focuses on cell counting and identification, chemical analyses, significance of lab findings and microbiological tests for meningitis. Brain anatomy and physiology and the production of CSF are also reviewed. Version Date: November 2011

Code 9804-11
Level Basic

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Additional Details

PEP hours: 16
CPS credits: 0
Level: Basic
Course Type: Express

Start Date: Upon registration
Completion: Up to 52 weeks
Delivery: PDF via email

Prerequisites: None
Textbook: N/A
Equipment: Computer with Internet is required

Learning Outcomes:

  • Draw and describe the major anatomical sites of the brain and its relationship to CSF, function, production and flow.
  • Outline the procedure for lumbar puncture and obtaining CSF.
  • Describe the choice of samples for testing CSF in different sections of the lab.
  • Describe the characteristics appearance of normal and commonly seen abnormal characteristics seen in CSF samples.
  • Describe the routine procedure for testing CSF.
  • Discuss the appearance of cells found in CSF in normal situations and common abnormal situations.
  • List, with their descriptions, the cells commonly referred to as lining cells.
  • Explain how occasionally cells, such as cartilage, epithelial and bone marrow, occur in a CSF sample.
  • Describe the features which are typical of malignant cells.
  • List the common malignancies which metastasize and result in the presence of malignant cells in the CSF.
  • Define meningitis and encephalitis.
  • State the importance and limitations of the gram stain in a patient with meningitis.
  • Describe the stains used to examine CSF.
  • Describe in detail the organisms which cause bacterial meningitis.
  • Outline the agents associated with viral, fungal and parasitic meningitis.
  • Tabulate the Normal (Reference Values) Ranges of the commonly measured chemistry tests.
  • Describe the interpretation of abnormal results from common chemistry tests.
  • Describe the commonly used tumor markers to determine the origin of malignant cells.
  • Discuss the value of testing a CSF with serological tests for syphilis.
  • Describe the routine to determine if a nasal drainage specimen contains CSF.
  • Discuss some of the newer technologies that are being used in investigating meningeal disease.

Author/Instructor: John Chapman, FCSMLS, FIMLS, CLSp(H)
Version Date: November 2011